Web引用和文献. Click-iT™ RNA Alexa Fluor™ 488 成像试剂盒能够检测细胞和组织内总体 RNA 转录的时间和空间变化 (PNAS (2008) 105:15779-84)。. 检测新合成的 RNA 或疾病、环 …
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WebOct 5, 2024 · For detection of nascent RNA, cells were incubated with 1 mM EU 30 min before fixation and EU detection was performed using Click-iT RNA imaging kit (Invitrogen, C10329) according to manufacturer ... WebClick-iT® RNA Imaging Kits Catalog nos. C10329, C10330 Introduction The Click-iT® RNA Imaging Assay enables detection of global RNA synthesis temporally and spatially …
WebOct 8, 2024 · Exosome-packaged RNA labeling was performed using a Click-iT RNA Imaging Kit according to the manufacturer’s instructions (C10329, Invitrogen). Briefly, CAFs were labeled with 100 μM 5-EU for 24 h, and LUAD cells were labeled with Dil for 10 min. Both cells types were then washed and co-cultured for 8 or 24 h. Webcopy, Live-cell imaging 1 Introduction Genome integrity is constantly challenged by endogenous, as well as exogenous sources of DNA damage including replication stress, reactive cellular metabolites, UV, ionizing radiation, and other mutagens including various chemicals. These threats generate a
WebIndeed, it worked very well. After we ran out of the kit, we made our own buffer (1x, 48mM Tris, 39mM glycine, 0.1% SDS, ph 9.2, 20% methanol), and used WYPALL X60 Wipers as paper stacks. I ... Web(C10329) Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit - Thermo Scientific - CiteAb. Kit [C10329] Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit by Invitrogen Antibodies. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact; ... Life Technologies, Pierce, Gibco, Applied Biosystems, Affymetrix ...
WebOct 19, 2024 · The nascent RNA was visualized using the Click-iT RNA Alexa Fluor 488 Imaging Kit (Molecular Probes, Invitrogen, C10329). Mounting was done using ProLong Diamond Antifade Mountant with DAPI (P36962, Life Technologies). 3i CSU-W1 Spinning disk (objective 40×, 63× or 100×) microscope was used for obtaining images.
WebInvitrogen Click It Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, … headlands county park michiganWebThe Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84). The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage or drug treatments is an important aspect of toxicological ... gold milanese apple watch bandWebMillipore pig rabbit millipore sigma sab4500548 Pig Rabbit Millipore Sigma Sab4500548, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. headlands cottages pacific city oregonWebFeb 3, 2010 · The ability to detect newly synthesized RNA or global changes in RNA levels in a temporal manner is an important aspect of molecular biology; important changes in … gold milgrain ringWebNov 14, 2024 · The transferred EU-labelled RNAs were tracked by Alexa Fluor 488 azide (Included in Click-iT® RNA Imaging Kit; Invitrogen, C10329). After 24 h co- culture of endometrial cells with EU-labelled spheroids, the conditioned media was removed and the endometrial cells were incubated with pre-warmed cell tracker working solution for 30 min ... headlands crossword clueWebPublish. - Specifications - Invitrogen. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian česk ... Click. Discover. Publish. - Specifications - Invitrogen . Click. Discover. Publish. - Specifications - Invitrogen ... gold migration lawyers reviewsWebSimilar to BRD4, we observed that H4K12ac occupancy increases near the transcription start sites (TSS) of estrogen-induced genes as well as at distal ERα binding sites in an estrogen-dependent manner. Interestingly, H4K12ac occupancy highly correlates with BRD4 binding and enhancer RNA production on ERα-positive enhancers. Consistent with an i... gold milanese apple watch