WebOct 18, 2024 · An isotype control is an antibody selected as a negative control for flow cytometry experiments, and is used to detect non-specific binding. Isotype controls can … WebMouse IgG1 kappa Isotype Control Antibodies Isotype control antibodies are similar to the primary antibody but are raised against an antigen that is not present in the experimental sample. They should match the host species, antibody class, and subclass, and conjugate of the primary antibody.

FITC Mouse IgG1, κ Isotype Ctrl Antibody - BioLegend

WebThe isotype control serves two purposes. Firstly, It would confirm the specificity of your primary antibody. Secondly, it will show you the fluorescence resulting from FC receptor mediated... WebThe isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues. Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). Recommended Usage old refrigerator commercial

Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21

WebIdeally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. WebIsotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant … WebIf you are using a mouse IgG1 monoclonal antibody that is conjugated to FITC, you should select a mouse IgG1 isotype control conjugated to FITC. As the fluorophore conjugation to the antibody (known as the F/P ratio) can vary between suppliers, it is best to purchase the isotype from the same supplier as the primary. my ny online service