WebInvitrogen™ Flp-In™ T-REx™ 293 Cell Line. Cells contain single stably integrated FRT site at transcriptionally active genomic locus. Includes: 1 x 10 7 cells are supplied frozen in 1mL of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. WebThe Flp-In™ T‑REx™-293 cell line is derived from 293 human embryonic kidney cells (Graham et al., 1977). The 293 parental cell line was obtained from the American Type Culture Collection (ATCC®). For more information about the 293 parental cell line, see …
Why I observe different growth curves in Flp-In T-REx system?
WebFeb 4, 2024 · inducible cell lines to express either hnRNPA1 wild type or D262V mutant epitope-tagged cDNAs. These cells were generated using FLP-recombinase mediated integration in 293 cells, while expressing endogenous wild type hnRNPA1. This experimental set up was initially designed to mimic ALS patients carrying a heterozygous … WebApr 1, 2016 · Symbols and abbreviations are as described in Fig. 1. (E) Flp-In-293 cells were transiently transfected with the indicated GPR37 constructs for 24 h, and the anti-Myc-antibody-labelled full-length receptors were analysed by confocal microscopy as … hamline anderson hours
Transmembrane helices 1 and 6 of the human breast cancer …
WebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture … WebThe cell line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA. A portion of human adenovirus 5, nucleotides (nts) 1-4344, is integrated into chromosome 19 of the HEK cells. Human adenovirus 5 causes a range of respiratory illnesses. HEK 293 cell lines are commonly used in cancer research. WebRNA-seq of the NE cells homozygous for W1282X showed that CFTR transcript level was reduced to 1.7% of WT (p-value: 4.6e-3). Negligible truncated CFTR protein was generated by Flp-In 293 cells stably expressing the W1282X EMG even though CFTR transcript was well above levels observed in the parents and proband. burn test chart