2024 Pef6/v5-his b

Pef6/v5-his b

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WebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one … WebRK2载体价格3800元，RK2载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄、卡那、四环素，质粒大小为56 kb。RK2载体是质粒。

pEF6/V5-His A, B, C - Thermo Fisher Scientific

WebVector Data. Vivid colors pcDNA6.2/C-EmGFP/GW/CAT . Restriction; Sequence; Map; Vector Data. pENTR L1-pLac-lacZalpha-L4 WebMoss (National Institutes of Health, Bethesda, MD). The pEF6/V5-His-TOPO® expression vector and the monoclonal mouse anti-V5 antibody were purchased from Invitrogen. Genomic DNA isolated from periph-eral blood lymphocytes of healthy European-, African-, Chinese-, and Hispanic-American volunteers was purchased from Coriell Cell Repos- boxing classes in midland tx

RM57043 invitrogen 价格表【第15001条-第16000条】-化工仪器网

http://www.labbase.net/Supply/SupplyItems-4229347.html WebpEF6/V5-His A, B, and C are 5.8 kb vectors derived from pcDNA™6/V5-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors … WebDescription pEF-DEST51 is a 7.5 kb vector derived from pEF6/V5-His and adapted for use with the Gateway ™ Technology. It is designed to allow high-level, constitutive expression in a variety of mammalian hosts. Features pEF-DEST51 contains the following elements: • Human elongation factor 1α boxing classes in nairobi

pEF6/V5-His A, B, and C - Gene Synthesis - yumpu.com

pEF6 V5-His B Sequence and Map - SnapGene

WebThe pEF6/V5-His-TOPO® expression vector and the monoclonal mouse anti-V5 antibody were purchased from Invitrogen. Genomic DNA isolated from peripheral blood lymphocytes of healthy European-, African-, Chinese-, and Hispanic-American volunteers was purchased from Coriell Cell Repositories (Camden, NJ). An enzymatic deglycosylation kit was ... http://www.biovector.net/product/44170.html boxing classes in orderWebAll pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the … gurps undead template

"Webwere cloned separately into the pEF6 V5-His-TOPO mammalian expression vector (Invitrogen), and these plasmids were transfected into susceptible C3H He cells with Lipofectamine 2000 (Life Technologies, Grand Island, NY). Stable integrants were selected by using blasticidin S, and cells from individual foci were isolated with cloning rings and ... " - Pef6/v5-his b

Pef6/v5-his b

WebThermo Fisher mammalian expression pef6 v5 his topo plasmid vector Mammalian Expression Pef6 V5 His Topo Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia …

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WebpEF6/V5-His (Search Vector Database) Backbone manufacturer Invitrogen Backbone size w/o insert (bp) 5840 Total vector size (bp) 6531 Vector type Mammalian Expression Selectable markers Blasticidin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown … WebInvitrogen™ pEF6/V5-His A, B, & C Mammalian Expression Vectors Contains strong promoter for high-level expression in mammalian cells Manufacturer: Invitrogen™ V96120

WebpEF6-V5-His (Search Vector Database) Backbone size w/o insert (bp) 5840 Total vector size (bp) 8009 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) … Web[CANCER RESEARCH 64, 7690–7696, November 1, 2004] Advances in Brief Activin Type II Receptor Restoration in ACVR2-Deficient Colon Cancer Cells Induces Transforming Growth Factor- Response Pathway Genes

WebDec 8, 2006 · HEK-293T cells were transfected with pEF6/V5-His recombinant vector containing Lrmp (A), Casc1 (B), Ghiso (C), or Lmna-rs1 (D) full-length cDNAs, grown for 48 h, fixed with paraformaldehyde, and treated with anti-V5 monoclonal antibody followed by secondary antibody-Alexa Fluor 488 conjugate. WebJan 15, 2013 · Full-length ADAMTS4 has a protumorigenic effect, whereas full-length E362A inhibits B16 melanoma tumor growth. (a) Expression of ADAMTS4 full-length proteins—WT and E362A mutant in conditioned medium of stably transfected B16 melanoma cell lines using pEF6/V5-His TOPO vector analyzed by immunoblotting with anti-V5 antibody. Blot …

WebJun 2, 2014 · WT and mutant SOX11 cDNAs were PCR amplified and cloned into the pEF6/V5-His B mammalian expression vector (Life Technologies) using the In-Fusion PCR Cloning Kit (Clontech Laboratories), and also ...

WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian český русский български العربية Unknown gurps vampire the masquerade pdfWebiv Kit Contents and Storage Shipping and Storage pVAX1™ and pVAX1™/lacZ are shipped at room temperature. Upon receipt, store vectors at –20°C. Kit Contents 20 µg each of … boxing classes in phillyWebpEF6 V5-His TOPO® TA Vector Kit pEF6/V5-His TOPO® TA Vector Kit, included in the MembranePro™ Functional Protein Expression Kit and also available separately (see page 29), contains the reagents listed below. Upon receipt, store the vector kit at –20°C. Reagent Composition Amount pEF6/V5-His-TOPO® vector 10 ng/µL plasmid DNA 20 µL boxing classes in san joseWebDensitometric analysis of (A) LRP6 phosphorylation and (B) total LRP6 content in cells treated with the indicated concentrations of Rspo2-2F, Rspo2-2F/Q70R and/or Wnt-3a for 1, 6 or 20 h prior to processing for immunoblotting. Cell lysates also were immunoblotted for HSP70 as a loading control. gurps warriors pdfWebInvitrogen™ pEF6/V5-His A, B, & C Mammalian Expression Vectors. Contains strong promoter for high-level expression in mammalian cells. Manufacturer: Invitrogen™ … gurps using bio tech to make a werewolfWebAug 14, 2015 · zt321 pIZT/V5-His 载体 Invitrogen 昆虫细胞表达载体系统. zt322 pMIB v5-His B 载体 Invitrogen 昆虫细胞表达载体系统. zt323 pFBDM 载体 Invitrogen 昆虫细胞表达载体系统. zt324 pUCDM 载体 Invitrogen 昆虫细胞表达载体系统. zt325 pBADZ-His6Cre 载体 Invitrogen 昆虫细胞表达载体系统 gurps warriorWebpEF6 V5-His A Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5-His B or pEF6/V5-His … boxing classes in pretoria